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KMID : 1134820110400070949
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Journal of the Korean Society of Food Science and Nutrition
2011 Volume.40 No. 7 p.949 ~ p.955
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Macrophage and Anticancer Activities of Feed Additives on β-Glucan from Schizophyllum commune in Breast Cancer Cells
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Lee Jin-Seok
Lee Seung-Ho
Jang Yong-Man
Lee Jong-Dae
Lee Byoung-Hee
Jung Ji-Youn
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Abstract
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β-Glucan is a polysaccharide expressed on the cell walls of fungi. It is known that β-glucan is recognized by a family of C-type lectin receptors, dectin-1, which is expressed mainly on myeloid immune cells, including macrophages, neutrophils and dendritic cells. Raw 264.7 cells were treated with β-glucan from Schizophyllum commune. β-Glucan was not cytotoxic up to 400 ㎍/㎖ as measured by MTT assay. To measure the activity of macrophages, NO and TNF-α assays were performed in Raw 264.7 cells. Treatment with β-glucan for 24hr significantly increased production of NO and TNF-α compared with control groups (p＜0.05), indicating activation of macrophages. To measure inhibition of breast cancer cell proliferation, MTT assay was performed in MDA-MB-231 cells. Cell viability was significantly decreased in the group treated with 400 ㎍/㎖ of β-glucan for 48 hr (p＜0.05) compared to the control group. However, tumor volume was decreased in the groups administered 200 ㎍ of β-glucan/mouse compared to the control group. These results indicate that β-glucan inhibits breast cancer cell growth through the induction of apoptosis.
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KEYWORD
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β-glucan, macrophage, anticancer, breast cancer, Schizophyllum commune
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